Single-stranded DNA-dependent ATP-dependent helicase. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair. When associated with KU80, binds to double-stranded telomeric and non-telomeric DNA sequences, but not to single-stranded DNA.

Ku70 and Ku80 make up the Ku70/80 heterodimer. They are encoded by the Xrcc5 and Xrcc6 genes, respectively, and are highly abundant in both prokaryotes and eukaryotes. The stability of these proteins depends on each other.

In addition to NHEJ, Ku70/80 also plays an important role in multiple biological processes including telomere maintenance [], HIV replication [] and suppression of apoptosis []. Ku70 and Ku80 form a pseudosymmetrical heterodimer with a preformed ring (also called DNA aperture) responsible for sequence‐independent DNA binding. Invitrogen Anti-Ku70/Ku80 Monoclonal (162), Catalog # MA1-21818. Tested in Immunocytochemistry (ICC/IF), Immunohistochemistry (Paraffin) (IHC (P)) and Dec 5, 2006 Accumulation of XRCC4/ligase IV on DSBs depended on the presence of Ku70/ 80, but not DNA-PKCS. We detected a direct interaction between Feb 27, 2014 The KU70-deficient mutant generated herein was effective in improving gene deletion frequency and allowed shorter homology sequences to Monoclonal Antibody for studying Ku70 in the Cell Cycle / Checkpoint research area. Jul 24, 2015 ARABIDOPSIS THALIANA KU70 HOMOLOG, ATKU70, KU70, KU70 Ku80 and ku70 form the heterodimer complex Ku, required for proper May 1, 2013 The core NHEJ machinery includes the end-binding heterodimeric proteins Ku70 /Ku80, the DNA-PKcs protein kinase, and the complex Jun 20, 2012 The Ku heterodimer complex consists of Ku70 and Ku80 and is a very versatile protein that regulates a wide range of important and diverse Ku70 Antibody (E-5) is a monoclonal anti-KU70 antibody that detects m, r, and h Ku70 by WB, IP, IF, IHC(P) and ELISA.

Ku70

Ruta 252 – Utländskt skatteregistreringsnummer (TIN) Observera! Dessa uppgifter finns inte med i e-tjänsten Lämna kontrolluppgifter. Ku70 and Ku80 make up the Ku70/80 heterodimer. They are encoded by the Xrcc5 and Xrcc6 genes, respectively, and are highly abundant in both prokaryotes and eukaryotes. The stability of these proteins depends on each other. Ku70 is an evolutionarily conserved protein that has functions in DNA repair and maintenance [96].

I de fallen ska royaltyn alltid redovisas på KU70. Ku70 and Ku80 make up the Ku70/80 heterodimer.

Because Ku70 sequesters Bax from mitochondria, cells overexpressing Bax alone are more susceptible to apoptosis than are cells overexpressing both Bax and Ku70. In the presence or absence of Ku70 overexpression, 293T cells grown in serum from CR animals were less susceptible to Bax-mediated apoptosis as compared to cells grown in media with AL serum ( Fig. 1D ).

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Ku70 or Ku80 overexpression by transfection with the Ku70 or Ku80 expression gene, respectively, enhanced proliferation of cells with low NF-κB levels. These

Supplied as 100 µL purified antibody (0.25 mg/mL). 2021-04-04 Ku70 plus Ku80 double knockout potently inhibits LPS-induced production of pro-inflammatory cytokines in human macrophages In order to exclude the possible off-target effect of the applied Ku70 and Ku80 shRNAs, we established the CRISPR/Cas9-gene editing methods to complete knockout Ku70 and Ku80. As described, the lenti- Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70. The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex to form the core of the non-homologous end joining (NHEJ) complex. 2001-03-01 Invitrogen Anti-Ku70 Recombinant Monoclonal (JM61-31), Catalog # MA5-32645. Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (Flow) and Immunoprecipitation (IP) applications. This antibody reacts with Human samples.

Invitrogen Anti-Ku70 Polyclonal, Catalog # PA5-27538. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), Immunohistochemistry (Paraffin) (IHC (P)), Immunoprecipitation (IP) and Proximity Ligation Assay (PLA) (PLA) applications. This antibody reacts with Human samples. Supplied as 100 µL purified antibody (0.25 mg/mL). 2021-04-04 Ku70 plus Ku80 double knockout potently inhibits LPS-induced production of pro-inflammatory cytokines in human macrophages In order to exclude the possible off-target effect of the applied Ku70 and Ku80 shRNAs, we established the CRISPR/Cas9-gene editing methods to complete knockout Ku70 and Ku80.
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This plasmid is available through Addgene. Invitrogen Anti-Ku70 Polyclonal, Catalog # PA5-27538. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), Immunohistochemistry (Paraffin) (IHC (P)), Immunoprecipitation (IP) and Proximity Ligation Assay (PLA) (PLA) applications.

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Immunogen Synthetic peptide corresponding to Human Ku70 aa 550 to the C-terminus conjugated to keyhole limpet haemocyanin. (Peptide available as ab92421)

In vivo, Ku70 -/- mice, known to be defective in B- but not T-lymphocyte maturation, developed thymic and disseminated T-cell lymphomas at a mean age of 6 months with CD4+/CD8+ tumor cells. Ku70 (also known as XRCC6) is a eukaryotic protein that is involved in the repair of DNA double-strand breaks by non-homologous end-joining [ 1, 2 ] Ku is a heterodimer of approximately 70kDa and 80kDa subunits The Ku70/Ku80 heterodimer has ATP-dependent DNA helicase activity and functions as the DNA-binding regulatory component of DNA-dependent protein kinase (DNA-PK) (6-8). The assembly of the DNA-PK complex at DNA ends is required for nonhomologous end-joining (NHEJ), one mechanism involved in double-stranded DNA break repair and V(D)J recombination (8). KU70 antibody Rabbit Polyclonal from Proteintech validated in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC),Enzyme-linked Immunosorbent Assay (ELISA) applications.

Kontrolluppgiftsblanketter. Det finns ett flertal olika kontrolluppgiftsblanketter i inkomstslaget tjänst: KU10 (lön och förmåner m.m.), KU13 (SINK), KU14 (pensionsgrundande inkomst m.m.), KU16 (sjöinkomst), KU17 (SINK med sjöinkomst), KU18 (pensions- och försäkringsutbetalare) och KU19 (pensions- och försäkringsutbetalare med SINK).

Because Ku70 sequesters Bax from mitochondria, cells overexpressing Bax alone are more susceptible to apoptosis than are cells overexpressing both Bax and Ku70.

Significantly, LPS-induced NFκB activation was inhibited by Ku70 plus Ku80 double knockdown or DKO. It was however enhanced with Ku70 and Ku80 overexpression. The Ku heterodimer (Ku70 and Ku80 subunits) contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the non-homologous end-joining pathway. The crystal structure of the human Ku heterodimer was determined both alone and bound to a 55-nucleotide DNA element at 2.7 and 2.5 A resolution, respectively. ku70 tert : gl1; ku70; tert: images; None available : phenotypes ; Surovtseva YV, Shakirov EV, Vespa L, Osbun N, Song X, Shippen DE(2007) In contrast to the ku70 mutant, which undergo telomerase-dependent expansion to more than twice the normal length in a single generation, the ku70 tert double mutant displays accelerated telomere shortening and a precocious onset of genome stability. 2021-02-26 · Depletion of CBP and/or Ku70 inhibited cell growth and caused cell death. To understand the roles of CBP and Ku70 in human melanoma cells, we designed and synthesized a set of CBP siRNA and Ku70 Although the ku70 deletion strain was noted to be more sensitive to UV rays than the corresponding wild-type strain, no lethality, severe growth retardation or loss of gene copy numbers could be detected during repetitive rounds of cultivation and induction of heterologous protein production. 2021-04-04 · Ku70 A-31G polymorphism is involved in the etiology of RCC; Ku70-1310C/G promoter polymorphism is associated with cancer; Hypoxia-induced cell lethality was facilitated by DNKu70, but substantially repaired upon reoxygenation.